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+Be certain all of the purification buffers are at 4 °C and perform the protein purification within the chilly room to avoid protein denaturation. NOTE: This avoids the coupling of amino acids in the additional cycles with the membrane as a substitute of the rising peptide chain. WORD: After the coupling of the first C-terminal amino acid of all peptides to the amino group of the membrane, the amino groups between the spots and in addition a few of the amino teams throughout the spot areas do not type bonds with the amino acids.
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